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KMID : 0380020060210020129
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 2 p.129 ~ p.132
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Antimicrobial Activity of Water-soluble Extract from Artemisia princeps var. orientalis
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Cho Hwa-Young
Yoon Sung-Yong
Park Jeong-Jin
Yun Kung-Won
Park Jong-Moon
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Abstract
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The importance of natural preservative has increased in recent years. The natural preservatives have been used in the field of foods, cosmetics and pharmacology. In the present work Artemisia sp., well recognized for their effect of antimicrobial activity, were extracted by methanol and water sequentially for selecting only water-soluble compounds that can be used as additives in food and cosmetics. Antimicrobial activities of water extracts from stem and leaf of Artemisia princeps var. orientalis were investigated by the disc diffusion method. Two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and three gram negative bacteria (Escherichia coil, Agrobacterium tumefaciens and Pseudomonas putida) were used for antimicrobial activity studies. The water-soluble compounds from methanol extract showed higher antimicrobial activity than only water extract to these bacteria. Comparative evaluation of water-soluble metabolite profiles with caffeic acid that is known as an antimicrobial compound from Artemisia sp. was performed by high performance liquid chromatography with photo-diode array detection.
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KEYWORD
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Antimicrobial activity, Artemisia princeps var. orientalis, caffeic acid, disc diffusion method, Staphylococcus aureus
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